The Following Projects are Taking Place at the ChEMTox Laboratory:
DNA Interaction and Mutational Activity of Novel Anticancer Drugs (Benzazolo[3,2-a] quinolinium Salts)
The main goal of this NIH funded project project is to characterize the DNA binding capacity and mutational activity of four novel bioreductive anticancer drugs. These novel compounds have synthesized belongs to a new family of cationic alkaloids known as benzazolo[3,2-a]quinolinium salts (BQS's). Dr. Zayas´s group have been working on the caharacterization of the mechanism of action of these potentail anti cancer agents. The analysis of DNA adduct formation is also being study with the aplication of high performance liquid chromatography mass spectrometry (HPLC-MS) to characterize the nucleoside interaction capacity. The cell toxicity, apoptosis induction and caspases activation of these drugs are being analyzed with tumor cells in culture. Further studies are being performed to better described the binding of these cationic compunds with key cellular organelles such as mitochondria and DNA
Extraction and Identification of Polycyclic Aromatic Hydrocarbons from sediments and plants of the Cucharillas Marshland, Cataño, Puerto Rico.
This study will determine the presence of PAH in sediments of the Cucharillas wetland by developing an extraction and characterization method using High Performance Liquid Chromatography and Mass Spectrometry (HPLC-MS).
Cytotoxic Effects of BQ’s Compounds on Normal Human Lymphocytes Cells in culture
This study presents the toxicity and mitochondrial interactions of five benzazolo [3,2-a]quinolinium salts (BQ’s) on normal TK-6 lymphocytes and a preliminary comparison with A431 tumor cells .
Tested drugs were NBQ38, ABQ38, NBQ95 and ABQ95. For determination of the IC50 (inhibition concentration) TK-6 cultures were exposed to BQ’s for 48 hours and cytotoxicity assessed by Trypan Blue exclusion. Apoptosis induction through mitochondrial membrane permeability was also determined. Current study includes membrane permeability and Caspases activation on TK6 determined by fluorescent analysis. Mitochondrial permeability analysis with TK-6 is being performed at this stage.
Cytotoxic Study: Effects of Di-ethylhexyl Phthalate and Mono ethylhexyl Phthalate on a Normal Human Lymphocytes Cell Line
Cytotoxic Study: Effects of Di-ethylhexyl Phthalate and Mono ethylhexyl Phthalate on a Normal Human Lymphocytes Cell Line The main objective of this study was to determine the toxicity of the phthalates Di-ethylhexyl (DEHP) and its principal metabolite, Mono-ethylhexyl (MEHP) on the TK-6 human lymphoblast cell line. Phthalates are a family of compounds used widely in the manufacturing industry and in the production of plastics. Exposure to these compounds can be through personal and consumer items such as health care and beauty products. Are considered estrogen disrupters but also have been observed effects over the respiratory systems by the scientific community. Studies related with the phthalates exposure have been performed however are limited as well in human cells.
Plasticizer principal metabolite (2 Ethyl-1-Hexanol) effects over the human lymphoblast TK6 cells
The main objective of this study is to determine the toxicity of the 2-Ethyl-1-Hexanol on the TK6 human lymphoblast cell line. The 2-Ethyl-1-hexanol (EH) is an organic alcohol produced in the cells as result of the biotransformation of di-ethylhexyl phthalate (DEHP). The exposure of humans to the compound 2-Ethyl-1-hexanol can be through environmental sources such as paint lacquer, inks, rubber, dry cleaning and plasticizers, e.g. PVC. .
Determination of Carcinogenic Compounds (PAH) in Tilapia using the microwave extraction method
This project aims to isolate PAH from tissues of Tilapia (Oreochromis mossambicus). This goal will be achieved by developing tissue extraction methods and characterization by High Performance Liquid Chromatography and Mass Spectrometry (HPLC-MS).
"The projects described was supported in part by Grant Number P20 RR-016470 from the National Center for Research Resources (NCRR), a component of the National Institutes of Health (NIH). Its contents are solely the responsibility of the authors and do not necessarily represent the official views of NCRR or NIH."
These investigations were conducted in the Laboratory ChEMTox that this belongs to the School Environmental Affairs.